Effect of D-penicillamine pre- and post-implantation treatment on formation of sponge-induced granulation tissue in rats

摘要: DNA, collagen and sulfated glycosaminoglycans (GAGs) of rat sponge granulation tissue were studied after 42 days D-penicillamine (D-pen) treatment at 100, 250 or 500 mg/kg/day, starting 10 before 28 induction formation. The effects compared with those observed when the same dosage was started onset formation pair-fed controls. D-pen stimulated cell invasion into implants, as manifested by an increased DNA content, particularly pre-treatment. Pre-treatment reduced net deposition per assessed lower hydroxyproline/DNA ratio, dose leading to concentration. total amount remained essentially unaffected within observation period. Salt-soluble augmented in a dose-dependent manner, irrespective protocol, signifying decreased cross-linking. Both pre- post-implantation enhanced radiosulfate incorporation GAGs, regardless dose, whereas from day implantation had no effect. It is concluded that enhances early connective response injury. Reduction cross-linking deposition, concomitant stimulation proteoglycan metabolism, may pertain antirheumatoid activity D-pen. observations suggest long-term administration D-pen, new attacks arthritis, be most effective controlling developing articular fibrosis.