Essential Requirement of Antigen Presentation by Monocyte Lineage Cells for the Activation of Primary Human γδ T Cells by Aminobisphosphonate Antigen

摘要: Human gammadelta T cells respond to nonpeptide Ags such as pyrophosphomonoesters and alkylamines in a TCR-dependent manner the absence of other APCS: Recently, aminobisphosphonates pamidronate have also been shown activate human cells. In present study, we indicate that activation primary by strictly depends on presence monocyte-lineage cells, unlike pyrophosphomonoesters. Thus, although induced cell clustering, proliferation, IFN-gamma production culture PBMC, it failed induce any these activities purified By adding back monocytes, however, both clustering could be restored. The pamidronate-pulsed, but not untreated, myelomonocytic line, THP-1, was capable activating produce IFN-gamma, which associated with down-regulation TCR. Furthermore, pamidronate-pulsed THP-1 were significantly more susceptible cell-mediated cytotoxicity than untreated THP-1. Also, TCR-defective Jurkat transfected TCR genes produced significant level IL-2 response These results suggested strongly are functionally activated via aminobisphosphonate Ag presented surface monocyte lineage rather directly its free form.