A DNA vaccination regime including protein boost and electroporation protects cattle against foot-and-mouth disease.

摘要: Abstract Protection against foot-and-mouth disease (FMD) using DNA technology has been documented for sheep and pigs but not the highly susceptible species of cattle. Twenty-five Holstein Friesian cross-bred cattle were vaccinated twice, 21 days apart, with a vaccine containing capsid coding region (P1) along non-structural proteins 2A, 3C 3D (pcDNA3.1/P1-2A3C3D) O 1 Kaufbeuren alone or coated onto PLG ( d , l -lactide-co-glycolide) microparticles. In some pcDNA3.1/P1-2A3C3D was also combined an adjuvant plasmid expressing bovine granulocyte macrophage colony stimulating factor (GM-CSF). vaccinations administered intramuscularly with, without, use electroporation at 42 days post primary vaccination received protein boost 146S FMD virus (FMDV) antigen 3D. For comparison, four conventional two more included as unvaccinated controls. Apart from those immunised microparticles all challenged 10 5 TCID 50 adapted Lausanne FMDV day 93 vaccination. All regardless regime developed good humoral cell mediated responses prior to challenge. The best overall neutralising antibody, IFN-γ clinical protection (75%) seen in whereby delivered by electroporation. contrast, only 25% without clinically protected. addition GM-CSF combination further improved efficacy vaccine, demonstrated reduction excretions nasal swabs. We thus demonstrate first time that can be protected challenge following prime-protein strategy, particularly when is