A eutherian-specific microRNA controls the translation of Satb2 in a model of cortical differentiation.

摘要: Cerebral cortical development is controlled by key transcription factors that specify the neuronal identities in different layers. The mechanisms controlling their expression distinct cells are only partially known. We investigated and stability of Tbr1, Bcl11b, Fezf2, Satb2, Cux1 mRNAs single developing mouse cells. observe Satb2 mRNA appears much earlier than its protein a set broader expected, suggesting an initial inhibition translation, subsequently released during development. Mechanistically, 3'UTR modulates translation GFP reporters corticogenesis. select miR-541, eutherian-specific miRNA, miR-92a/b as best candidates responsible for SATB2 inhibition, being strongly expressed early reduced late progenitor Their inactivation triggers robust premature both human Our findings indicate RNA interference major mechanism timing cell identities.