New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models.
作者： Isabel Ortiz , Jesús Dorado , Jane Morrell , Jaime Gosálvez , Francisco Crespo
DOI: 10.1186/S40104-017-0155-7
关键词: Semen 、 Sperm washing 、 Linear regression 、 Centrifugation 、 Molecular biology 、 Fragmentation (cell biology) 、 Sperm 、 Biology 、 Biophysics 、 DNA fragmentation 、 Coefficient of determination
摘要: Sperm DNA fragmentation (sDF) has been proved to be an important parameter in order predict vitro the potential fertility of a semen sample. Colloid centrifugation could suitable technique select those donkey sperm more resistant after thawing. Previous studies have shown that elucidate latent damage molecule, sDF should assessed dynamically, where rate between treatments indicates how is iatrogenic damage. The calculated using slope linear regression equation. However, it not studied if dynamics fit this model. objectives study were evaluate effect different after-thawing protocols on and most accurate mathematical model (linear regression, exponential or polynomial) for over time frozen-thawed semen. After submitting post-thaw samples no (UDC), washing (SW) single layer (SLC) protocols, values 6 h incubation significantly lower SLC than SW UDC. Coefficient determination (R2) higher second polynomial exponential. highest acceleration (aSDF) obtained SW, followed by thawing seems preserve longer longevity comparison UDC SW. Moreover, fine-tuning models model, which implies constant must taken into account hidden molecule.
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Sarah P. Otto, Troy Day, A Biologist's Guide to Mathematical Modeling in Ecology and Evolution ,(2007)
Sheena E.M. Lewis, Should sperm DNA fragmentation testing be included in the male infertility work-up? Reproductive Biomedicine Online. ,vol. 31, pp. 134- 137 ,(2015) , 10.1016/J.RBMO.2015.05.006
Sezgin Gunes, Maha Al-Sadaan, Ashok Agarwal, Spermatogenesis, DNA damage and DNA repair mechanisms in male infertility Reproductive Biomedicine Online. ,vol. 31, pp. 309- 319 ,(2015) , 10.1016/J.RBMO.2015.06.010
J. Gosálvez, R. Núñez, J. L. Fernández, C. López-Fernández, P. Caballero, Dynamics of sperm DNA damage in fresh versus frozen-thawed and gradient processed ejaculates in human donors. Andrologia. ,vol. 43, pp. 373- 377 ,(2011) , 10.1111/J.1439-0272.2010.01022.X
F Crespo, L Gutiérrez-Cepeda, J Gosalvez, C Serres, SD Johnston, Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation. Reproduction in Domestic Animals. ,vol. 48, ,(2013) , 10.1111/J.1439-0531.2012.02140.X
A. Colin Cameron, Frank A.G. Windmeijer, An R-squared measure of goodness of fit for some common nonlinear regression models Journal of Econometrics. ,vol. 77, pp. 329- 342 ,(1997) , 10.1016/S0304-4076(96)01818-0
Burak Ozkosem, Sheldon I. Feinstein, Aron B. Fisher, Cristian O’Flaherty, Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice Redox biology. ,vol. 5, pp. 15- 23 ,(2015) , 10.1016/J.REDOX.2015.02.004
J. Gosalvez, J.L. Fernandez, A. Gosalbez, F. Arrollo, A. Agarwal, C. Lopez-Fernandez, Dynamics of sperm DNA fragmentation in mammalian species as assessed by the SCD methodology Fertility and Sterility. ,vol. 88, pp. S365- ,(2007) , 10.1016/J.FERTNSTERT.2007.07.1215
C. López-Fernández, F. Crespo, F. Arroyo, J.L. Fernández, P. Arana, S.D. Johnston, J. Gosálvez, Dynamics of sperm DNA fragmentation in domestic animals II. The stallion. Theriogenology. ,vol. 68, pp. 1240- 1250 ,(2007) , 10.1016/J.THERIOGENOLOGY.2007.08.029
J. Gosálvez, M.A. Ramirez, C. López-Fernández, F. Crespo, K.M. Evans, M.E. Kjelland, J.F. Moreno, Sex-sorted bovine spermatozoa and DNA damage: I. Static features Theriogenology. ,vol. 75, pp. 197- 205 ,(2011) , 10.1016/J.THERIOGENOLOGY.2010.08.006