作者: J. Ehrenfeld , J. -L. Cousin
DOI: 10.1007/BF01871588
关键词: Membrane 、 Permeability (electromagnetism) 、 Cyanide 、 Dunaliella 、 Biology 、 Monensin 、 Ion transporter 、 Osmoregulation 、 Compartmentalization (fire protection) 、 Chromatography 、 Analytical chemistry
摘要: Different techniques were investigated in order to determine the Na, K and Cl concentrations ofDunaliella tertiolecta cells adapted a large range of salinity (20 1640 mM NaCl). The cell 6 13 times higher than concentration external medium (11 mM). Na concentrations, on other hand, lower at all salinities tested. Considerable differences absolute values were, however, found according technique employed. These results are interpreted terms compartmentalization (at least two compartments). It is postulated that larger compartment regulates its ion maintaining low high whereas second equilibrates with medium. cation permeability membrane limiting regulating altered by antibiotics nystatin monensin. Incubation K-free leads decrease an increase this effect being reversed addition KCl A good correlation between gain loss suggesting stoichiometric exchange these ions. magnitude apparent Na/K increases as increases. necessary mediate half-saturation function NaCl adaptation This partially light-dependant inhibited cold, cyanide DCCD. suggested mechanism helps regulation ionic composition cells.