Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region

摘要: We present a three-color multiplex coherent anti-Stokes Raman scattering (CARS) setup that facilitates prompt recording of broadband CARS spectra along with fast imaging. With separate narrowband Stokes and probe beams being introduced in the near IR, we are able to incorporate stable, wideband Ti:sapphire femtosecond laser as pump beam covers full range shift for CHn stretching vibrational modes. Experimentally, high-resolution signals allowed investigate molecular vibrations over 2650 cm-1–3050 cm-1, which spectrally integrated construct lipid-sensitive images. It is demonstrated proposed implementation promises particular benefit on imaging lipid-rich tissue structures by providing detailed information Raman-active at points interest images can be obtained high frame rates.