Protective effect of toll-interacting protein overexpression against paraquat-induced lung injury in mice and A549 cells through inhibiting oxidative stress, inflammation, and NF-κB signaling pathway

摘要: Toll-interacting protein (Tollip) is a pivotal negative regulator of inflammatory response. In the present study, effects Tollip overexpression on paraquat (PQ)-induced lung injury were explored through in vivo and vitro investigations. Upon stimulation with PQ mice, expression was down-regulated. Histopathological analysis revealed that significantly decreased cell infiltration. Similarly, levels myeloperoxidase (MPO) interleukin-1β (IL-1β) lowered by PQ-administrated mice. Besides, reduced reactive oxygen species (ROS) generation malondialdehyde (MDA) level but enhanced superoxide dismutase (SOD) activity PQ-treated A549 cells. Meanwhile, IL-1β expressions p-p65 cytoplasm nuclear p65. Importantly, inhibition NF-κB signaling pathway probably decreasing p65-DNA binding induced overexpression. Taken together, attenuated PQ-initiated possibly via reduction oxidative stress inflammation suppression activation, which provided some novel ideas for treatment damage mediated PQ.