Preferential Activation of CD4+Vβ5.2/5.3+Intestinal Intraepithelial Lymphocytes in the Inflamed Lesions of Crohn's Disease

摘要: Abstract To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory disease, we established T cell lines iIELs isolated from endoscopic biopsied ileal and colonic mucosa Crohn's disease patients. Seven inflamed terminal ileum 13 patients, but none 16 normal ileum, have shown a deviation receptor variable region gene usage were enriched proportion CD4 + Vβ5.2/5.3 cells. cells not increased after stimulation with purified protein derivatives or 65-kDa heat-shock significantly staphylococcal enterotoxins C1 D. Those showed cytolytic activity against target cross-linked by anti-Vβ5.2/5.3 produced large amount interferon-γ. These results indicated that preferentially activated lesions may play possible role triggering progression human disease.