Bacterial cellulose nano crystal as hydrocolloid matrix in immobilized β-galactosidase onto silicon dioxide nanoparticles
作者： Samira Tizchang , Mahmood Sowti Khiabani , Reza Rezaei Mokarram , Hamed Hamishehkar
DOI: 10.1016/J.LWT.2020.109091
关键词: Environmental pollution 、 Bacterial cellulose 、 Entrapment 、 Fourier transform infrared spectroscopy 、 Hydrolysis 、 Immobilized enzyme 、 Enzyme assay 、 Nuclear chemistry 、 Matrix (chemical analysis) 、 Chemistry
摘要: Abstract β-galactosidase immobilization was optimized on silicon dioxide nanoparticles for the hydrolysis of lactose using Design Box-Behnken. Under conditions, exhibited efficiency 87% and enzyme activity 3.4 (U). Results entrapment showed that maximum obtained in ratio: bacterial cellulose nano crystal to immobilized (1:2). Optimum pH free 6 after shifted 6.5. The optimal temperature 40 °C but reached 50 °C. Entrapment increased Km decreased Vmax comparison with enzyme. result indicated higher stability entrapped form 45 days storage at 4 °C. Entrapped retained about 80% its initial 12 cycles use. Also, results FTIR, XRD, SEM, TEM AFM confirmed entrapment. As a result, as novel productive method can improve efficacy order decrease environmental pollution costs food processing.
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