Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects of Cassia alata L.
作者： M. Ouédraogo , F. L. Da , A. Fabré , K. Konaté , C. I. Dibala
DOI: 10.1155/2013/162651
关键词: Cassia 、 Glibenclamide 、 Muscarinic acetylcholine receptor 、 Genotoxicity 、 In vivo 、 Propranolol 、 Pharmacology 、 Medicine 、 Methylene blue 、 Comet assay
摘要: Aqueous-ethanolic extract of Cassia alata (AECal) and its derived fractions obtained through liquid-liquid fractionation were evaluated for their bronchorelaxant, genotoxic, antigenotoxic effects. Contractile activity rats’ tracheas in the presence tested materials, as well modifications with different inhibitors blockers, was isometrically recorded. The potential AECal on cyclophosphamide- (CP-) induced genotoxicity rat. Animals pretreated extract, then liver comet assay performed. chloroformic (CF-AECal) relaxed contraction by Ach, but both significantly less potent inhibiting KCl (30 mM; 80 mM). Propranolol, indomethacin, L-NAME, methylene blue, glibenclamide did not modify relaxant effect CF-AECal. TEA altered response trachea to CF-AECal caused a rightward shift without affecting cumulative concentration-response curves Ach only at low concentrations. In animals percentage CP-induced DNA damage decreased. Our results suggest that (1) muscarinic receptors contribute least part effects CF-AECal; (2) interferes membrane polarization; (3) is genotoxic vivo contains chemopreventive phytoconstituents offering protection against genotoxicity.
hindawi.com
archives-ouvertes.fr参考文章(48)
XU Lizhen, Zou Zhongmei, Yang Shilin, Liu An, Studies on chemical constituents from leaves of Cassia alata China journal of Chinese materia medica. ,vol. 34, pp. 861- 863 ,(2009)
Nielsen-Kudsk Je, POTASSIUM CHANNEL MODULATION : A NEW DRUG PRINCIPLE FOR REGULATION OF SMOOTH MUSCLE CONTRACTILITY : STUDIES ON ISOLATED AIRWAYS AND ARTERIES Danish Medical Bulletin. ,vol. 43, pp. 429- 447 ,(1996)
C. P. Khare, Indian Medicinal Plants: An Illustrated Dictionary ,(2007)
Irene M. Villasenor, Sonia D. Jacinto, Annabelle A. Herrera, Elizabeth I. Olarte, Antioxidant activity of a new indole alkaloid from Cassia alata L. Philippine Agricultural Scientist. ,vol. 93, pp. 250- 254 ,(2010)
T B Bolton, Mechanisms of action of transmitters and other substances on smooth muscle. Physiological Reviews. ,vol. 59, pp. 606- 718 ,(1979) , 10.1152/PHYSREV.1979.59.3.606
C.P. Khare, Comprar Indian Medicinal Plants · An Illustrated Dictionary | Khare, C.P. | 9780387706375 | Springer Springer. ,(2007)
M Noroozi, W J Angerson, M E Lean, Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. The American Journal of Clinical Nutrition. ,vol. 67, pp. 1210- 1218 ,(1998) , 10.1093/AJCN/67.6.1210
Farhana Masood, Reshma Anjum, Masood Ahmad, Abdul Malik, Methods for Genotoxicity Testing of Environmental Pollutants Springer, Dordrecht. pp. 229- 260 ,(2012) , 10.1007/978-94-007-1591-2_7
Matthias Sausbier, Xiao‐Bo Zhou, Caroline Beiern, Ulrike Sausbier, Daniela Wolpers, Sylvi Maget, Christian Martin, Alexander Dietrich, Anna‐Rebekka Ressmeyer, Harald Renz, Jens Schlossmann, Franz Hofmann, Winfried Neuhuber, Thomas Gudermann, Stefan Uhlig, Michael Korth, Peter Ruth, None, Reduced rather than enhanced cholinergic airway constriction in mice with ablation of the large conductance Ca2+-activated K+ channel The FASEB Journal. ,vol. 21, pp. 812- 822 ,(2007) , 10.1096/FJ.06-7167COM
Shelly Tartakover Matalon, Asher Ornoy, Michael Lishner, Review of the potential effects of three commonly used antineoplastic and immunosuppressive drugs (cyclophosphamide, azathioprine, doxorubicin on the embryo and placenta). Reproductive Toxicology. ,vol. 18, pp. 219- 230 ,(2004) , 10.1016/J.REPROTOX.2003.10.014