Chemical Composition of Exosporium from Spores of Bacillus cereus

作者: L. L. Matz , T. Cabrera Beaman , Philipp Gerhardt

DOI: 10.1128/JB.101.1.196-201.1970

关键词: ExosporiumMethionineRhamnoseBacillus cereusPolysaccharideBiochemistryBiologyAmino acidAmino sugarGlucosamineMolecular biologyMicrobiology

摘要: Homogeneous fragments of exosporium were extricated in centigram amounts from dormant spores Bacillus cereus and analyzed for intrinsic constituents. The membrane proved to be chemically complex but not unique, consisting mainly protein (52%), amino neutral polysaccharides (20%), lipids (18%), ash (4%). Seventeen common acids identified by chromatography, present usual proportions except low levels cystine-cysteine, methionine, tyrosine, histidine. Glucosamine was the only sugar, glucose rhamnose principal sugars. lipid fraction contained 5.5% cardiolipin as phospholipid, 12.5% lipids, at least 19 fatty acids, among which normal C(16) C(18) ones predominated. Calcium phosphorus occurred ash. Small teichoic, ribonucleic, dipicolinic believed represent contamination.

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