Organization and functional analysis of the mouse transporter associated with antigen processing 2 promoter.

摘要: In accordance with the key role of MHC class I molecules in adaptive immune response against viruses, they are expressed by most cells, and their expression can be enhanced cytokines. The assembly cell surface complexes depend on a continuous peptide supply. peptides generated mainly proteasome transported to endoplasmic reticulum transport pump consisting two subunits, TAP1 TAP2. low molecular weight polypeptide (2 7), as well TAP (1 2) genes, coordinately regulated induced IFNs. Despite this coordinate regulation, examination tumors shows that these genes discordantly down-regulated. pursuing explanation for observations, we have characterized mouse TAP2 promoter region 5'-flanking sequence. We show 5' untranslated regions characteristic genomic organization is conserved both human. belongs promoters lack TATA boxes but contain MED1 (multiple start site element downstream) Accordingly, transcription initiated from multiple sites within 100-nucleotide window. An IFN regulatory factor 1 (IRF1)/IRF2 binding located involved basal IRF1-induced activity. implication extensive differences found among heavy chain, polypeptide, all encoding proteins Ag presentation, discussed.