Functionalized 3D-hydrogel plugs covalently patterned inside hydrophilic poly(dimethylsiloxane) microchannels for flow-through immunoassays.
作者： Wang-Chou Sung , Huang-Han Chen , Honest Makamba , Shu-Hui Chen
DOI: 10.1021/AC901138W
关键词: Copolymer 、 Polydimethylsiloxane 、 Microfluidics 、 Nanotechnology 、 Chemistry 、 Photoinitiator 、 Protein G 、 Substrate (chemistry) 、 Covalent bond 、 Polyelectrolyte
摘要: Integration of a hydrogel and polydimethylsiloxane (PDMS)-based microfluidic device can greatly reduce the cost developing channel-based devices. However, there are technical difficulties including hydrophobic inert surface properties associated with PDMS as well back pressure fragile material use in microchannels. In this study, strategy to covalently photopattern 3-D plugs functionalized protein G inside channels on hydrophilic substrate coated polyelectrolyte multilayers (PEMS) is presented. process, UV-light microscope applied initiate G−poly(acryl amide) copolymerization from bulk solution areas via deeply implanted photoinitiator (PI), resulting sturdy 3D bonded upper lower channel wall, while leaving open spaces width for fluid flow through. addition, long-term hydrophilicity low nonspecific bindin...
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