Population genomics of the pathogenic yeast Candida tropicalis identifies hybrid isolates in environmental samples.

摘要: Abstract Candida tropicalis is a human pathogen that primarily infects the immunocompromised. Whereas genome of one isolate, C. MYA-3404, was originally sequenced in 2009, there have been no large-scale, multi-isolate studies genetic and phenotypic diversity this species. Here, we used whole sequencing phenotyping to characterize 77 isolates from clinical environmental sources variety locations. We show most are diploids with approximately 2 - 6 heterozygous variants per kilobase. The genomes relatively stable, few aneuploidies. However, identified highly homozygous isolate six much higher heterozygosity levels ranging 36 49 Our analyses represent two different hybrid lineages, where hybrids share parent (A) other isolates, but second (B or C) differs by at least 4% level. Four descend an AB hybridization, AC hybridization. MTLa/α heterozygotes. Hybridization, mating, between parents therefore common evolutionary history tropicalis. new were predominantly found niches, including soil. Hybridization unlikely be associated virulence. In addition, genotype-phenotype correlation CRISPR-Cas9 editing identify variant results inability utilize certain branched-chain amino acids as sole nitrogen source. Author summary important fungal pathogen, which particularly Asia-Pacific Latin America. There currently very little known about genotype phenotype isolates. By carrying out phylogenomic analysis find range heterozygous. hybrids, likely formed mating parents. Unlike species, more than suggesting for hybridization not also explore phenotypes, genomic required growth on valine isoleucine sources.