Design and fabrication of microstructured and switchable biological surfaces

摘要: The research presented in this thesis explores the design and fabrication of microstructured switchable biological surfaces, which may have potential applications nanobiotechnology. focuses on surfaces can be controlled via external stimuli. Chapter 1 - Introduction to Nanobiotechnology presents an introduction background including role self-assembled monolayers (SAMs) nanobiotechnology, microstructure techniques, stimuli responsive cell migration. 2 Surface characterization techniques surface employed throughout research. 3 Study Arp2/3 complex activity filopodia spreading cells using patterned (fibronectin) patterning technology (microcontact printing) several analytical techniques. This study Mouse Embryonic fibroblast (MEF) function process. results demonstrated that filopodia, produced by MEF interacted with fibronectin guided lamellipodia protrusion. complex, is absent adhesion site, does not facilitate surface. 4 Tuning specific biomolecular interactions electro-switchable oligopeptide rely peptides control gold surfaces. system based upon conformational switching positively charged oligolysine are tethered a bioactive molecular moieties (biotin) terminates oligolysines reversibly exposed (bio-active state) or concealed (bio-inactive demand, as potential. 5 Experimental procedures, protocols synthesis describes experimental used during investigations performed work described thesis. data analysis various equipment described.