A novel human cell culture model for the study of familial prostate cancer.

摘要: Research into molecular and genetic mechanisms underlying familial prostate cancer would be greatly advanced by in vitro models of tumor cells representing primary tumors. We have successfully established an immortalized human epithelial cell culture derived from tumors patients with telomerase. The actively proliferating early-passaged 957E were transduced through infection a retrovirus expressing the telomerase catalytic subunit, reverse transcriptase (hTERT). A high level activity was detected 957E/hTERT cells, but not cells. are currently growing well at passage 40, whereas senesced 5. exhibit morphology. Expression androgen-regulated specific homeobox gene NKX3.1 cell-specific cytokeratin 8, antigen or androgen receptor, Prostatic stem p16 also expressed this line. showed growth inhibition when exposed to retinoic acid transforming factor β1, potent inhibitors growth. Chromosome analysis that line (passage 10) near diploid male (XY), most chromosome counts 44–46 range. However, there random loss chromosomes 13, X, Y, alteration 4q. late 32) karyologically similar early had same 4q observed as trisomy 20. well-characterized lines such will useful for identification characterization susceptibility genes. This is first documented case patient.