Regulation of proteoglycan and hyaluronan synthesis by elevated level of intracellular cyclic adenosine monophosphate in peritubular cells from immature rat testis.
作者： Bénédicte Thiébot , Monique Langris , Pierre-Jacques Bonnamy , Jean Bocquet
关键词: Chondroitin sulfate 、 Chemistry 、 Proteoglycan 、 Molecular biology 、 Hyaluronan synthase 、 Glycosaminoglycan 、 Cholera toxin 、 Biochemistry 、 Cyclic adenosine monophosphate 、 Intracellular 、 Heparan sulfate
摘要: The effects of an increase in intracellular cAMP concentration on proteoglycan (PG) synthesis by peritubular (PT) cells from immature rat testis were investigated. In the presence dBcAMP for 72 h, [3H]-hexosamine incorporation secreted PG and cellassociated was reduced, whereas [35S]-sulfate radioactivity enhanced not affected cell-associated PG. Cholera toxin IBMX, known to generate high levels, induced similar changes. Cyclic AMP did alter protein moiety but turnover. profoundly modified characteristics: (1) Apparent molecular weight increased. (2) This due glycosaminoglycans (heparan sulfate (HS) chondroitin (CS)) length. (3) number glycosaminoglycan chains presumably reduced. (4) Heparan medium cell layer-associated appeared oversulfated. (5) pattern layer associated with a decrease HSPG correlative CSPG. also dramatically stimulated hyaluronan possible phosphorylation activation synthase(s).
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