A PTK7/Ror2 Co-Receptor Complex Affects Xenopus Neural Crest Migration

摘要: Neural crest cells are a highly migratory pluripotent cell population that generates wide array of different types and failure in their migration can result severe birth defects malformation syndromes. is controlled by various means including chemotaxis, repellent guidance cues cell-cell interaction. Non-canonical Wnt PCP (planar polarity) signaling has previously been shown to control cell-contact mediated neural guidance. PTK7 (protein tyrosine kinase 7) transmembrane pseudokinase known regulator Wnt/PCP signaling, which expressed Xenopus required for migration. functions as co-receptor; however, it remains unclear affects Expressing fluorescently labeled proteins we find co-localizes with the Ror2 Wnt-receptor. Further, co-immunoprecipitation experiments demonstrate interacts Ror2. The PTK7/Ror2 interaction likely relevant migration, because expression rescue loss function defect. Live imaging explanted shows formation protrusions well motility. Co-expression these defects. In vivo analysis demonstrates dead mutant cannot function. Thus, our data suggest substitute its domain this effect.