NADH-Mediated Gene Expression in Streptococcus pneumoniae and Role of Rex as a Transcriptional Repressor of the Rex-Regulon
作者： Muhammad Afzal , Sulman Shafeeq , Oscar P. Kuipers
关键词: Chemistry 、 Operator (biology) 、 Operon 、 Regulon 、 Gene expression 、 Mutant 、 Gene 、 Cell biology 、 NAD+ kinase 、 Regulatory sequence
摘要: Nicotinamide adenine dinucleotides (NAD(H)) play a vital role in various biological processes, including keeping the cellular redox balance. In this study, we investigate regulatory responses of Streptococcus pneumoniae D39 to NADH and characterize Rex protein as transcriptional repressor gapN, fba, pncB, adhB2, gap, adhE genes. Transcriptomic analysis was used observe response S. NADH. Our microarray studies revealed elevated expression genes/operons involved transport biosynthesis niacin (gapN, adhE). Promoter-lacZ fusion assays with mutant uptake biosynthesis, presence We predict operator site (5’-TTGTKAWAAWWTTCACAA-3’) regions Rex-regulated genes that subsequently validated by promoter mutational experiments.
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