Independent expression of the two HL-A antigen polypeptide chains.

摘要: It is now well established that beta2-microglobulin constitutes one of the two HL-A antigen subunits. In this study support was obtained for previous notion human lymphoma Daudi does not produce (beta2m). Papain-solubilized as Nonidet P-40-solubilized antigens do contain any beta2m or detectable structural analogue protein. The chemical and physico-chemical characteristics highly purified derived from cells are indistinguishable those antigen-carrying polypeptide chain isolated P3HRIK cell line. Like P3HRIK-derived antigens, composed identical heavy, alloantigenic chains with a molecular weight about 50 000 each, which held together by disulfide bridge(s). contain, in contrast to molecules beta2m. Although no evidence suggesting synthesis it apparent these express amounts similar other lines present data suggest [1] under separate genetic regulation [2], surface integration independent presence [3] constitute membrane component absolutely necessary integrity membrane.