A NOVEL MOBILE MONITORING SYSTEM FOR FAST AND AUTOMATED BACTERIA DETECTION IN WATER

作者: Ulrich Reidt , Leonhard Meixner , Christoph Heller , Hans Wolf , Andreas Helwig

DOI:

关键词: FluorescenceFluidicsMaterials scienceMonitoring systemMicroorganismFluorescence spectroscopyBiosensorNanotechnologyFilter (video)Bacteria

摘要: Keywords: micromechanical filters, micro fluidic system, fluorescence detection, bacteria sampling, potable water. Abstract: Standard detection methods for viable in water are time consuming due to a required cultivation step. Fast and automated of borne microorganisms with high sensitivity selectivity is still challenge. We report on novel biosensor using filters nano sized pores capture enrich the filter surface subsequent fluorescent probes. The whole process fully by integrating sieves into system together performance detector. results show effective retention surface, which then accessible different staining procedures. As an example, we use special dyes that bind or intercalate DNA molecules bacteria. After microfilters undergo cleaning conditioning steps be ready next measurement.

参考文章(10)
E. Ogura, P.J. Abatti, T. Moriizumi, Measurement of human red blood cell deformability using a single micropore on a thin Si/sub 3/N/sub 4/ film IEEE Transactions on Biomedical Engineering. ,vol. 38, pp. 721- 726 ,(1991) , 10.1109/10.83583
Xing Yang, Joon Mo Yang, Yu-Chong Tai, Chih-Ming Ho, Micromachined membrane particle filters Sensors and Actuators A-physical. ,vol. 73, pp. 184- 191 ,(1999) , 10.1016/S0924-4247(98)00269-6
Tzung K. Hsiai, Sung Kwon Cho and, Joon Mo Yang, Xing Yang and, Yu-Chong Tai, Chih-Ming Ho, Pressure Drops of Water Flow Through Micromachined Particle Filters Journal of Fluids Engineering-transactions of The Asme. ,vol. 124, pp. 1053- 1056 ,(2002) , 10.1115/1.1514209
Chieko Sakamoto, Nobuyasu Yamaguchi, Masao Nasu, Rapid and simple quantification of bacterial cells by using a microfluidic device. Applied and Environmental Microbiology. ,vol. 71, pp. 1117- 1121 ,(2005) , 10.1128/AEM.71.2.1117-1121.2005
ULRICH REIDT, LINA CHAUHAN, GERHARD MÜLLER, RAMONA MOLZ, PETRA LINDNER, HANS WOLF, ALOIS FRIEDBERGER, REPRODUCIBLE FILTRATION OF BACTERIA WITH MICROMECHANICAL FILTERS Journal of Rapid Methods and Automation in Microbiology. ,vol. 16, pp. 337- 350 ,(2008) , 10.1111/J.1745-4581.2008.00140.X
Kiev S Gracias, John L McKillip, A review of conventional detection and enumeration methods for pathogenic bacteria in food. Canadian Journal of Microbiology. ,vol. 50, pp. 883- 890 ,(2004) , 10.1139/W04-080
E. Ogura, B. Kusumoputro, T. Moriizumi, Passage time measurement of individual and blood cells through arrayed micropores on Si3N4 membrane Journal of Biomedical Engineering. ,vol. 13, pp. 503- 506 ,(1991) , 10.1016/0141-5425(91)90099-S
Helena Aurell, Philippe Catala, Pierre Farge, France Wallet, Matthieu Le Brun, J�rgen H. Helbig, Sophie Jarraud, Philippe Lebaron, Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry. Applied and Environmental Microbiology. ,vol. 70, pp. 1651- 1657 ,(2004) , 10.1128/AEM.70.3.1651-1657.2004
John T. Lisle, Martin A. Hamilton, Alan R. Willse, Gordon A. McFeters, Comparison of Fluorescence Microscopy and Solid-Phase Cytometry Methods for Counting Bacteria in Water Applied and Environmental Microbiology. ,vol. 70, pp. 5343- 5348 ,(2004) , 10.1128/AEM.70.9.5343-5348.2004