The development of genome-wide single nucleotide polymorphisms in blue wildebeest using the DArTseq platform.

摘要: Abstract Blue wildebeest (Connochaetes taurinus taurinus) are economically important antelope that widely utilised in the South African wildlife industry. However, very few genomic resources available for blue can assist breeding management and facilitate research. This study aimed to develop a set of genome-wide single nucleotide polymorphism (SNP) markers wildebeest. The DArTseq genotyping platform, commonly used polyploid plant species, was selected SNP discovery. A limited number published articles have described use platform animals and, therefore, this also provided unique opportunity assess performance an animal species. total 20,563 SNPs, each located within 69 bp sequence, were generated. developed had high average scoring reproducibility (>99%) low percentage missing data (~9.21%) compared other reduced representation sequencing approaches been studies. Furthermore, candidate SNPs per position decreased towards 3′ end sequence reads, ratio transitions (Ts) transversions (Tv) remained similar read position. These observations indicate there no bias, such as identification false due quality, tail-end reads. successful identifying large informative with desirable parameters minor allele frequency (MAF). Bos taurus genome silico mapping marker sequences 6020 (29.28%) successfully mapped against bovine genome. all chromosomes establishing distribution SNPs. Moreover, observed Ts:Tv (2.84:1) targeted gene-rich regions Finally, functional annotation revealed wide range different putative functions indicating these be useful gene represents high-throughput, robust cost-effective which may find adoption several