CD74-dependent Deregulation of the Tumor Suppressor Scribble in Human Epithelial and Breast Cancer Cells

摘要: The γ subunit of the major histocompatibility complex (MHC) class II complex, CD74, is overexpressed in a significant proportion metastatic breast tumors, but mechanistic foundation and biologic significance this phenomenon are not fully understood. Here, we show that when CD74 human cancer noncancerous epithelial cells, it interacts interferes with function Scribble, product well-known tumor suppressor gene. Furthermore, using cell lines expressing under control tetracycline-inducible promoter quantitative high-resolution mass spectrometry, demonstrate that, as result overexpression, phosphorylation pattern C-terminal part Scribble undergoes specific changes. This accompanied translocation protein from sites cell-to-cell contacts at plasma membrane to cytoplasm, which likely effectively enhance motility invasiveness cells.