Phenolic Composition and Antioxidant and Antiproliferative Activities of the Extracts of Twelve Common Bean (Phaseolus vulgaris L.) Endemic Ecotypes of Southern Italy before and after Cooking

摘要: Beans are important dietary components with versatile health benefits. We analysed the extracts of twelve ecotypes Phaseolus vulgaris in order to determine their phenolic profiles, antioxidant activity, and vitro antiproliferative activity. Ultra-performance liquid chromatography diode array detector (UPLC-DAD) admitted us detect quantify some known polyphenols, such as gallic acid, chlorogenic epicatechin, myricetin, formononetin, caffeic kaempferol. The activity (AA) ranged from 1.568 ± 0.041 66.572 3.197 mg necessary inhibit 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by 50% (EC50). extracts, except those obtained nonpigmented samples, were capable inhibiting proliferation human epithelial colorectal adenocarcinoma (Caco-2) cells, breast cancer cells MCF-7, A549 NSCLC cell line. Cultivars differed composition concentration polyphenols including anthocyanins; cooking affected only marginally. Qualitative quantitative differences between groups beans influenced biological activities; on other hand, we did not find significant activities within same variety, before after cooking.