RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes.

作者: Yurong Xin , Jinrang Kim , Haruka Okamoto , Min Ni , Yi Wei

DOI: 10.1016/J.CMET.2016.08.018

关键词: TranscriptomeBiologyEndocrinologyCell biologyGene expression profilingPancreatic polypeptideIsletInternal medicineRNAGeneSingle-cell analysisType 2 diabetes

摘要: Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA …

参考文章(39)
Talitha van der Meulen, Cynthia J Donaldson, Elena Cáceres, Anna E Hunter, Christopher Cowing-Zitron, Lynley D Pound, Michael W Adams, Andreas Zembrzycki, Kevin L Grove, Mark O Huising, Urocortin3 mediates somatostatin-dependent negative feedback control of insulin secretion Nature Medicine. ,vol. 21, pp. 769- 776 ,(2015) , 10.1038/NM.3872
Peter Langfelder, Steve Horvath, WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics. ,vol. 9, pp. 559- 559 ,(2008) , 10.1186/1471-2105-9-559
Taman Mahdi, Sonja Hänzelmann, Albert Salehi, Sarheed J. Muhammed, Thomas M. Reinbothe, Yunzhao Tang, Annika S. Axelsson, Yuedan Zhou, Xingjun Jing, Peter Almgren, Ulrika Krus, Jalal Taneera, Anna M. Blom, Valeriya Lyssenko, Jonathan Lou S. Esguerra, Ola Hansson, Lena Eliasson, Jonathan Derry, Enming Zhang, Claes B. Wollheim, Leif Groop, Erik Renström, Anders H. Rosengren, Secreted Frizzled-Related Protein 4 Reduces Insulin Secretion and Is Overexpressed in Type 2 Diabetes Cell Metabolism. ,vol. 16, pp. 625- 633 ,(2012) , 10.1016/J.CMET.2012.10.009
Dominic Grün, Lennart Kester, Alexander van Oudenaarden, Validation of noise models for single-cell transcriptomics Nature Methods. ,vol. 11, pp. 637- 640 ,(2014) , 10.1038/NMETH.2930
Jalal Taneera, Joao Fadista, Emma Ahlqvist, Mengze Zhang, Nils Wierup, Erik Renström, Leif Groop, Expression profiling of cell cycle genes in human pancreatic islets with and without type 2 diabetes. Molecular and Cellular Endocrinology. ,vol. 375, pp. 35- 42 ,(2013) , 10.1016/J.MCE.2013.05.003
Jalal Taneera, Joao Fadista, Emma Ahlqvist, David Atac, Emilia Ottosson-Laakso, Claes B. Wollheim, Leif Groop, Identification of novel genes for glucose metabolism based upon expression pattern in human islets and effect on insulin secretion and glycemia Human Molecular Genetics. ,vol. 24, pp. 1945- 1955 ,(2015) , 10.1093/HMG/DDU610
J. Fadista, P. Vikman, E. O. Laakso, I. G. Mollet, J. L. Esguerra, J. Taneera, P. Storm, P. Osmark, C. Ladenvall, R. B. Prasad, K. B. Hansson, F. Finotello, K. Uvebrant, J. K. Ofori, B. Di Camillo, U. Krus, C. M. Cilio, O. Hansson, L. Eliasson, A. H. Rosengren, E. Renstrom, C. B. Wollheim, L. Groop, Global genomic and transcriptomic analysis of human pancreatic islets reveals novel genes influencing glucose metabolism Proceedings of the National Academy of Sciences of the United States of America. ,vol. 111, pp. 13924- 13929 ,(2014) , 10.1073/PNAS.1402665111
Martin Bengtsson, Anders Ståhlberg, Patrik Rorsman, Mikael Kubista, Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels Genome Research. ,vol. 15, pp. 1388- 1392 ,(2005) , 10.1101/GR.3820805
Frances M. Ashcroft, Patrik Rorsman, Diabetes Mellitus and the β Cell: The Last Ten Years Cell. ,vol. 148, pp. 1160- 1171 ,(2012) , 10.1016/J.CELL.2012.02.010