Spontaneous and radiation-induced apoptosis in lung carcinoma cells with different intrinsic radiosensitivities.

摘要: Spontaneous and radiation-induced apoptosis in three lung carcinoma cell lines (U-1285, U-1906 U-1810) with previously characterised intrinsic radiosensitivities (RS) was assessed by TUNEL-staining detection of DNA laddering cleavage poly-(ADP-ribose) polymerase (PARP). detected at a high level the radiosensitive U-1285, an intermediate U- 1906 not radioresistant U-1810 line. Radiation-induced apoptosis, TUNEL asssay, present U-1285 cells but cells. To explain these findings, expression Bcl-2, Bax, c-Myc RB protein mutations p53 gene were analysed. The ratio Bcl-2/Bax higher compared Overexpression loss found whereas both expressed showed lower expression. Analysis sequencing all exons disclosed lines. Thus, independent process overexpression may enhance Our data suggest that spontaneous correlate RS SCLC.