Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction.
作者： Xuexian O. Yang , Xiao-Dong Huang , Hua-Lu Zhao , Jun-Wu Zhang
DOI: 10.1007/BF02788681
关键词: Gene 、 Complementary DNA 、 Polymerase chain reaction 、 Biology 、 Rapid amplification of cDNA ends 、 Molecular cloning 、 Molecular biology 、 Cellular differentiation 、 cDNA library 、 Globin
摘要: An efficient and simple method for removing known, abundant cDNA species from the pool of highly differentiated cells is reported. The involves preparation sscDNA, followed by dscDNA-synthesis led specific primers removal synthesized dscDNA with hydroxyapatite. By using this method, globin cDNAs were reduced to less than 10(-5) their original abundance. results suggest that may facilitate isolation new genes or tissues.
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K Inoue, H Sugiyama, H Ogawa, M Nakagawa, T Yamagami, H Miwa, K Kita, A Hiraoka, T Masaoka, K Nasu, WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia. Blood. ,vol. 84, pp. 3071- 3079 ,(1994) , 10.1182/BLOOD.V84.9.3071.3071
U. B. Gyllensten, H. A. Erlich, Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 7652- 7656 ,(1988) , 10.1073/PNAS.85.20.7652
S. W. Lee, C. Tomasetto, R. Sager, Positive selection of candidate tumor-suppressor genes by subtractive hybridization. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 2825- 2829 ,(1991) , 10.1073/PNAS.88.7.2825
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
I. Wieland, G. Bolger, G. Asouline, M. Wigler, A method for difference cloning: gene amplification following subtractive hybridization Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 2720- 2724 ,(1990) , 10.1073/PNAS.87.7.2720
J Lederer, F Borriello, Construction of quantitative RT-PCR MIMICs. BioTechniques. ,vol. 19, pp. 580- 584 ,(1995)
T Furukawa, G Zitnik, K Leppig, T Papayannopoulou, G Stamatoyannopoulos, Coexpression of gamma and beta globin mRNA in cells containing a single human beta globin locus: results from studies using single-cell reverse transcription polymerase chain reaction [published erratum appears in Blood 1994 Aug 15;84(4):1357] Blood. ,vol. 83, pp. 1412- 1419 ,(1994) , 10.1182/BLOOD.V83.5.1412.1412