Turning tumor cells in situ into T-helper cell-stimulating, MHC class II tumor epitope-presenters: immuno-curing and immuno-consolidation

摘要: Abstract Immunological control or cure of tumors depends on initiating a robust T helper cell response to MHC class II epitopes tumor-associated antigens. cells regulate the potency cytotoxic lymphocyte and antibody responses. We have developed novel approach stimulate by converting tumor into molecule-positive, antigen presenting cells. Furthermore, using antisense methods, we suppress expression Ii protein, that normally blocks antigenic peptide binding site molecules during synthesis in endoplasmic reticulum. In such gene-engineered cells, pick up peptides, which been transported reticulum for I molecules. All nucleated create “surveys self” detect viral malignant transformation. Our method extends survey self endogenous Simultaneous presentation antigens both generates long-lasting antitumor immune response. Injecting murine with genes, induce cures significant number animals renal prostate tumors. analogous human gene vectors are suitable most patients cancers, because they monomorphic active all HLA-DR alleles. review our findings, analyze remaining issues preclinical study design clinical trials.