Identification of two new cytochrome P450 genes and RNA interference to evaluate their roles in detoxification of commonly used insecticides in Locusta migratoria.

摘要: Cytochrome P450 monooxygenases (cytochrome P450s), found in virtually all living organisms, play an important role the metabolism of xenobiotics such as drugs, pesticides, and plant toxins. We have previously evaluated responses oriental migratory locust (Locusta migratoria) to pyrethroid insecticide deltamethrin revealed that increased cytochrome enzyme activity was due transcription multiple genes. In this study, we identified for first time two new genes, which belong novel gene families. CYP409A1 belongs CYP409 family whereas CYP408B1 CYP408 family. Our molecular analysis indicated mainly expressed fatbodies, midgut, gastric caecum, foregut Malpighian tubules third- fourth-instar nymphs, foregut, hindgut muscle insects at developmental stages examined. The expression these genes were differentially affected by three representative insecticides, including carbaryl (carbamate), malathion (organophosphate) (pyrethroid). exposure carbaryl, resulted reduced, moderately significantly transcript levels, respectively, further their detoxification roles using RNA interference followed bioassay showed nymph mortalities 21.1% 16.7%, after silenced. These results strongly support our notion locust.