Role of two active site Glu residues in the molecular action of botulinum neurotoxin endopeptidase.
作者： Roshan V. Kukreja , Sapna Sharma , Shuowei Cai , Bal Ram Singh
DOI: 10.1016/J.BBAPAP.2006.11.007
关键词: Zinc 、 Enzyme 、 Active site 、 Site-directed mutagenesis 、 Chemistry 、 Endopeptidase activity 、 Endopeptidase 、 Residue (chemistry) 、 Glutamine 、 Stereochemistry
摘要: … fold decrease in catalytic efficiency. This mutation did not induce any significant structural alterations in the active site … Substitution of Glu262 with Gln however, dramatically impaired the …
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Bal Ram Singh, Intimate details of the most poisonous poison Nature Structural & Molecular Biology. ,vol. 7, pp. 617- 619 ,(2000) , 10.1038/77900
Identification of two active site residues in human angiotensin I-converting enzyme. Journal of Biological Chemistry. ,vol. 269, pp. 29430- 29434 ,(1994) , 10.1016/S0021-9258(18)43897-5
Jen Tsi Yang, Chuen-Shang C. Wu, Hugo M. Martinez, [11] Calculation of protein conformation from circular dichroism Methods in Enzymology. ,vol. 130, pp. 208- 269 ,(1986) , 10.1016/0076-6879(86)30013-2
Rakhi Agarwal, Thomas Binz, Subramanyam Swaminathan, Analysis of Active Site Residues of Botulinum Neurotoxin E by Mutational, Functional, and Structural Studies: Glu335Gln Is an Apoenzyme† Biochemistry. ,vol. 44, pp. 8291- 8302 ,(2005) , 10.1021/BI050253A
James G. Caya, Joan E. Miller, Rashmi Agni, Clostridium botulinum and the Clinical Laboratorian: A Detailed Review of Botulism, Including Biological Warfare Ramifications of Botulinum Toxin Archives of Pathology & Laboratory Medicine. ,vol. 128, pp. 653- 662 ,(2004) , 10.1043/1543-2165(2004)128<653:CBATCL>2.0.CO;2
Fen-Ni Fu, Richard B. Lomneth, Shuowei Cai, Bal Ram Singh, Role of zinc in the structure and toxic activity of botulinum neurotoxin. Biochemistry. ,vol. 37, pp. 5267- 5278 ,(1998) , 10.1021/BI9723966
O. Rossetto, P. Caccin, M. Rigoni, F. Tonello, N. Bortoletto, R.C. Stevens, C. Montecucco, Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity Toxicon. ,vol. 39, pp. 1151- 1159 ,(2001) , 10.1016/S0041-0101(00)00252-X
Liqing Zhou, Anton de Paiva, Dandan Liu, Roger Aoki, J. Oliver Dolly, Expression and Purification of the Light Chain of Botulinum Neurotoxin A: A Single Mutation Abolishes Its Cleavage of SNAP-25 and Neurotoxicity after Reconstitution with the Heavy Chain Biochemistry. ,vol. 34, pp. 15175- 15181 ,(1995) , 10.1021/BI00046A025
Li Li, Bal Ram Singh, High-level expression, purification, and characterization of recombinant type A botulinum neurotoxin light chain. Protein Expression and Purification. ,vol. 17, pp. 339- 344 ,(1999) , 10.1006/PREP.1999.1138
Bal Ram Singh, B.R. DasGupta, Changes in the molecular topography of the light and heavy chains of type A botulinum neurotoxin following their separation. Biophysical Chemistry. ,vol. 34, pp. 259- 267 ,(1989) , 10.1016/0301-4622(89)80063-8