Lack of correlation between Treg quantification assays in inflammatory bowel disease patients

摘要: AIM: To compare the number of regulatory T-cells (Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR (qPCR) method in patients suffering from inflammatory bowel disease (IBD). METHODS: Tregs percentages both and qPCR methods 35 adult IBD patients, 18 out them Crohn´s (CD) 17 ulcerative colitis (UC) were compared to each other as well scores on two activity questionnaires Harvey Bradshaw Index (HBI) for CD Simple Colitis Clinical Activity (SCCAI) UC patients. The Treg defined CD4+CD25highCD127lowFOXP3+ cells peripheral blood mononuclear cells, whereas determined FOXP3 promoter demethylation genomic DNA. RESULTS: We found an average 1.56% ± 0.78% cytometry, 1.07% 0.53% There no significant correlations between either or HBI SCCAI questionnaire respectively. In addition, there was correlation (r = -0.06, P 0.73; Spearman Rho). These data suggest that, Treg-related immune function clinical these did not accurately reflect actual activity. Until cause(s) differences are more clearly defined, results caution interpreting studies various disorders. CONCLUSION: produce equivalent measures percentage total studied which is consistent conclusion that subtypes equally detected assays.