The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris.
作者： Magdalena Frajnt , Małgorzata Cytryńska , Teresa Jakubowicz
关键词: Phosphodiesterase 3 、 Cell biology 、 Phosphorylation 、 Chemistry 、 Biochemistry 、 Pyruvate kinase 、 Enzyme 、 Protein kinase A 、 Protein phosphorylation 、 PDE10A 、 Pichia pastoris
摘要: Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of such as 8-chloro-cAMP and 8-bromo-cAMP were potent in PKA activation while N 6 ,2'-O-dibutyryl-cAMP did not stimulate the enzyme activity. It shown that protamine sulfate almost equally phosphorylated presence 1–2 10 –6 M cGMP other substrates Kemptide, ribosomal S 6, a lower extent demonstrated pyruvate is substrate which co-purified with P. enzyme. Moreover, comparable levels. plays key role regulation basic cellular pro
sci-hub.st 参考文章(23)
Gasior E, Szyszka R, Phosphorylation of ribosomal proteins during differentiation of Saccharomyces cerevisiae. Acta Biochimica Polonica. ,vol. 31, pp. 375- 382 ,(1984)
Teresa Jakubowicz, Phosphorylation-dephosphorylation changes in yeast ribosomal proteins S2 and S6 during growth under normal and hyperthermal conditions. Acta Biochimica Polonica. ,vol. 32, pp. 7- 12 ,(1985)
Dagfinn OGREID, Roald EKANGER, Robert H. SUVA, Jon P. MILLER, Priscilla STURM, Jackie D. CORBIN, Stein Ove DOSKELAND, Activation of protein kinase isozymes by cyclic nucleotide analogs used singly or in combination. Principles for optimizing the isozyme specificity of analog combinations. FEBS Journal. ,vol. 150, pp. 219- 227 ,(1985) , 10.1111/J.1432-1033.1985.TB09010.X
M J Zoller, J Kuret, S Cameron, L Levin, K E Johnson, Purification and characterization of C1, the catalytic subunit of Saccharomyces cerevisiae cAMP-dependent protein kinase encoded by TPK1. Journal of Biological Chemistry. ,vol. 263, pp. 9142- 9148 ,(1988) , 10.1016/S0021-9258(19)76518-1
J F Cannon, R Gitan, K Tatchell, Yeast cAMP-dependent protein kinase regulatory subunit mutations display a variety of phenotypes. Journal of Biological Chemistry. ,vol. 265, pp. 11897- 11904 ,(1990) , 10.1016/S0021-9258(19)38484-4
C.S. Hixson, E.G. Krebs, Characterization of a cyclic AMP-binding protein from bakers' yeast. Identification as a regulatory subunit of cyclic AMP-dependent protein kinase. Journal of Biological Chemistry. ,vol. 255, pp. 2137- 2145 ,(1980) , 10.1016/S0021-9258(19)86004-0
T Toda, S Cameron, P Sass, M Zoller, J D Scott, B McMullen, M Hurwitz, E G Krebs, M Wigler, Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 7, pp. 1371- 1377 ,(1987) , 10.1128/MCB.7.4.1371
Hans Eckstein, 3':5'-cyclic GMP in the yeast Saccharomyces cerevisiae at different metabolic conditions. FEBS Letters. ,vol. 232, pp. 121- 124 ,(1988) , 10.1016/0014-5793(88)80399-5
Malgorzata Cytrynska, Iwona Wojda, Magdalena Frajnt, Teresa Jakubowicz, PKA from Saccharomyces cerevisiae can be activated by cyclic AMP and cyclic GMP. Canadian Journal of Microbiology. ,vol. 45, pp. 31- 37 ,(1999) , 10.1139/W98-214
Takashi Toda, Scott Cameron, Philip Sass, Mark Zoller, Michael Wigler, Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase Cell. ,vol. 50, pp. 277- 287 ,(1987) , 10.1016/0092-8674(87)90223-6