Classification of the medicinal plants of the genusAtractylodesusing high-performance liquid chromatography with diode array and tandem mass spectrometry detection combined with multivariate statistical analysis

摘要: Analytical methods using high-performance liquid chromatography with diode array and tandem mass spectrometry detection were developed for the discrimination of rhizomes four Atractylodes medicinal plants: A. japonica, macrocephala, chinensis, lancea. A quantitative study was performed, selecting five bioactive components, including atractylenolide I, II, III, eudesma-4(14),7(11)-dien-8-one atractylodin, on twenty-six samples various origins. Sample extraction optimized to sonication 80% methanol 40 min at room temperature. High-performance established a C18 column water/acetonitrile gradient system flow rate 1.0 mL/min, wavelength set 236 nm. Liquid applied certify reliability results. The validated by ensuring specificity, linearity, limit quantification, accuracy, precision, recovery, robustness, stability. Results showed that cangzhu contained higher amounts I atractylodin than baizhu, especially contents greatest variation between baizhu cangzhu. Multivariate statistical analysis, such as principal component analysis hierarchical cluster also employed further classification plants. method suitable quality control