Candida albicans Amphotericin B-Tolerant Persister Formation is Closely Related to Surface Adhesion

摘要: Candida albicans persisters have so far been observed only in biofilm environment; the element(s) that trigger(s) persister formation are still unknown. In this study, we tried to further elucidate possible relationship between C. and early phases of formation, especially surface adhesion phase. Three strains were surveyed for persisters. We tested dynamically at different time points during process formation. The number cells was determined based on an assessment cell viability after amphotericin B treatment colony-forming unit assay. None planktonic cultures contained Immediately following surface, emerged proportion reached a peak 0.2–0.69 % approximately 2-h biofilm. As matured, decreased 0.01–0.02 % by 24 h, while remained stable with no significant change. Persisters not detected absence attachment which pre-coated. also absent biofilms scraped disrupt prior treatment. These results indicate antifungal-tolerant produced mainly phase is required emergence maintenance