Identification of drought responsive proteins using gene ontology hierarchy

摘要: The availability of the complete genome sequences has facilitated access to essential information identify proteins. determination Arabidopsis sequence had a great impact annotate data. sequencing Sorghum bicolor been only recently completed and hither global response abiotic stresses in this important crop remains largely unexplored. We used 2-D gel electrophoresis based proteomic approach refined with MALDI-TOF analyze drought-stress proteins sorghum. Major changes protein complement sorghum were observed hydroponic cultures at 96 hours under drought stress. Six most highly expressed excised for functional identification. Here, we developed method obtain distances between GO terms analyzed distance values allocate shortest path (SP) hierarchy. paths noted informative common ancestor (MICA) terms, viz. binding, catalytic activity primary metabolic process. belonged group signal transduction mechanisms, carbohydrate transport metabolism. These identified functions suggest different mechanism tolerant novel applied study may have importance further identifying involved biotic stress conditions crops.