Immunobiological characterization of Graphidium strigosum experimental infection in rabbits (Oryctolagus cuniculus).

摘要: An experimental infection of rabbits with a wild isolate the gastric nematode Graphidium strigosum was carried out. Animals (3.5 months age) were infected 1,000 L3 administered by bucoesophagic catheter (five rabbits) or kept as uninfected control group animals). The maintained for 3 months. Along period, some parasitological, hematological and immunological parameters determined. Prepatent period ranged from 30 to 38 days and, at necropsy, average adult helminth counts 430.75 ± 126.12. No significant variations found in packed cell volume, leukocyte, eosinophil along period. Infection elicited clear serum-specific IgG response, estimated ELISA, during patency. Pooled sera patent recognized soluble antigens, particularly, 67-kDa protein. Experimentally animals did not show cross recognition between G. strigosum, Haemonchus contortus, Teladorsagia circumcincta. However, Western blot analysis hyperimmune against H. contortus raised lambs showed reactivity this species strigosum.