Capillary LC coupled with high-mass measurement accuracy mass spectrometry for metabolic profiling.

摘要: We have developed an efficient and robust high-pressure capillary LC−MS method for the identification of large numbers metabolites in biological samples using both positive negative ESI modes. Initial efforts focused on optimizing separation conditions metabolite extracts various LC stationary phases conjunction with multiple mobile-phase systems, as applied to 45 standards. The optimal mobile those tested were determined experimentally (in terms peak shapes, theoretical plates, retention small, polar compounds, etc.), linear exponential gradients study from cyanobacterium Cyanothece sp. ATCC 51142. Finally, automated dual-capillary system was constructed evaluated effectiveness reproducibility chromatographic separations above samples. When coupled a commercial LTQ-orbitrap MS, ∼900 features reproducibly detected f...