The role of subcellular organelles in hormone secretion: The interactions of calcium, vitamin A, vinblastine, and cytochalasin B in PTH secretion

摘要: Abstract To determine the role of subcellular organelles in hormone secretion, we studied interaction low calcium concentration (low Ca), retinol (vitamin A, vit A), vinblastine (VB), and cytochalasin B (CB) parathyroid (PTH) secretion. Bovine tissue pieces were incubated media containing above agents. Vit A stimulated PTH release to a mean 170% control. This effect was diminished when tissues simultaneously with Ca and, furthermore, absent pre-incubated Ca. VB had no on Ca-stimulated but did inhibit A-induced secretion presence CB 150% control during second third hours incubation. at least an additive stimulating more prompt greater response than seen normal calcium. not acute media, CB-induced hour None agents lysosomal cathepsin D, stimulate LDH. Our results suggest that; (1) through common pathway involving cell membrane; (2) stimulates separate membrane or submembrane microfilaments, which normally retards PTH; (3) microtubular proteins may facilitate basal PTH, are involved PTH.