Packaging system for rapid production of murine leukemia virus vectors with variable tropism.

摘要: A method for rapidly producing helper-free murine leukemia virus (MLV) without using packaging cell lines is described. Viruses bearing ecotropic or amphotropic MLV Rous sarcoma envelope glycoprotein and containing various retroviral vector genomes have been prepared with titers 30 to 40-fold higher than those produced by transient transfection of standard cells. This system can be used alter the cellular tropism incorporating other glycoproteins prepare stocks establishing stable producer lines. will particularly useful preparing viruses that encode toxic proteins rapid analysis panels mutant glycoproteins.