Inhibition of anion transport in human red blood cells by 5,5′-dithiobis(2-nitrobenzoic acid)
DOI: 10.1016/0005-2736(83)90194-3
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摘要: The uptake of [32P]phosphate into human red blood cells was inhibited (Ki = 0.6 mM) by the sulfhydryl reagent 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). 2-Nitro-5-thiobenzoic acid (NTB), reduced form DTNB, a less potent inhibitor 7 mM). inhibition anion transport DTNB could be reversed washing DTNB-treated with isotonic buffer, or incubating 2-mercaptoethanol, which converted to NTB. competitively binding 4-[14C]-benzamido-4'-aminostilbene-2,2'-disulfonate, 1-2 microM), band 3 protein in and ghost membranes. These results suggest that stilbene-disulfonate site can readily accommodate organic not due reaction an essential group.
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sci-hub.se 参考文章(19)
Philip A. Knauf, Erythrocyte Anion Exchange and the Band 3 Protein: Transport Kinetics and Molecular Structure Current topics in membranes and transport. ,vol. 12, pp. 249- 363 ,(1979) , 10.1016/S0070-2161(08)60259-2
A Rao, Disposition of the band 3 polypeptide in the human erythrocyte membrane. The reactive sulfhydryl groups. Journal of Biological Chemistry. ,vol. 254, pp. 3503- 3511 ,(1979) , 10.1016/S0021-9258(18)50788-2
M K Ho, G Guidotti, A membrane protein from human erythrocytes involved in anion exchange Journal of Biological Chemistry. ,vol. 250, pp. 675- 683 ,(1975) , 10.1016/S0021-9258(19)41949-2
A Rao, R A Reithmeier, Reactive sulfhydryl groups of the band 3 polypeptide from human erythroycte membranes. Location in the primary structure. Journal of Biological Chemistry. ,vol. 254, pp. 6144- 6150 ,(1979) , 10.1016/S0021-9258(18)50530-5
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Michael L. Jennings, Hermann Passow, Anion transport across the erythrocyte membrane, in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonate. Biochimica et Biophysica Acta. ,vol. 554, pp. 498- 519 ,(1979) , 10.1016/0005-2736(79)90387-0
James A. Dix, A. S. Verkman, A. K. Solomon, L. C. Cantley, Human erythrocyte anion exchange site characterised using a fluorescent probe. Nature. ,vol. 282, pp. 520- 522 ,(1979) , 10.1038/282520A0
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
Saul Ship, Yehezkel Shami, William Breuer, Aser Rothstein, Synthesis of tritiated 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid ([3H]DIDS) and its covalent reaction with sites related to anion transport in human red blood cells. The Journal of Membrane Biology. ,vol. 33, pp. 311- 323 ,(1977) , 10.1007/BF01869522
Ian G. Macara, Lewis C. Cantley, Mechanism of anion exchange across the red cell membrane by band 3: interactions between stilbenedisulfonate and NAP-taurine binding sites. Biochemistry. ,vol. 20, pp. 5695- 5701 ,(1981) , 10.1021/BI00523A009