On the micro-indentation of plant cells in a tissue context

摘要: The effect of geometry on cell stiffness measured with micro-indentation techniques has been explored in single cells, however it is unclear if results cells can be readily transferred to indentation experiments performed a tissue vivo. Here we this question by using simulation models osmotic treatments and 3D multicellular tissues the finite element method. We found that cellular context does affect stiffness, several each direction are required for optimal results. applied model data obtained force microscopy sepal A. thaliana, differences could explained geometry, do not necessarily indicate wall material properties or turgor pressure.