Porcine malignant hyperthermia susceptibility: hypersensitive calcium-release mechanism of skeletal muscle sarcoplasmic reticulum.

摘要: Abstract This study tested the hypothesis that calcium-release from sarcoplasmic reticulum isolated malignant hyperthermia swine had abnormal concentration-dependency on release modulators. Halothane stimulated half-maximal at similar concentrations for and control (0.10 +/- 0.04 mM). However, causing were lower (P less than 0.001) by an order of magnitude Ca2+ (28.1 8.3 versus 1.23 0.45 nM), adenosine triphosphate (0.33 0.09 0.023 0.014 mM) caffeine (7.79 1.56 0.80 0.44 Half-maximal inhibition Mg2+ occurred threefold higher (0.23 0.02 0.78 0.17 The Ca2+-sensitivity curves heterozygotes hyperthermia-defect indistinguishable averages controls hyperthermia-homozygotes. Results this suggest is initiated due to a hypersensitive mechanism which inherited in autosomal, codominant pattern may be diagnosed using sensitivity-tests reticulum.