Tamm–Horsfall urinary glycoprotein. The subunit structure

摘要: 1. Subunit molecular weights of 76000–82000 were obtained for native and alkylated Tamm–Horsfall glycoprotein by gel filtration on Sephadex G-200 in the presence sodium dodecyl sulphate. 2. A further estimate subunit weight 79000±4000 was disc electrophoresis 3. minimum value chemical 79000±6000 from number N-terminal amino acids released cyanogen bromide cleavage glycoprotein. 4. Similar values patients with cystic fibrosis. 5. On ultracentrifugation both 1.0% sulphate 70% formic acid, sedimented as a single component, slightly faster than serum albumin. 6. reduction disulphide bonds same obtained, which suggested that these are intrachain.