Copper-induced oxidative damage to the prophenoloxidase-activating system in the freshwater crayfish Procambarus clarkii
作者： Keqiang Wei , Junxian Yang
DOI: 10.1016/J.FSI.2016.03.151
关键词:
摘要: Abstract Previous studies have demonstrated copper-induced proteins damage in gill and hepatopancreas of the freshwater crayfish Procambarus clarkii, but little information is available about its effects on key component innate defense haemolymph. In present study, we evaluated relationship between oxidative carbonylation prophenoloxidase-activating system (proPO-AS) activity, by exposing P. clarkii to sub-lethal concentrations (1/50, 1/12, 1/6 1/3 96 h LC50) Cu2+ up 96 h. Six biomarkers stress, i.e. reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), protein carbonyl (PC), malondialdehyde (MDA) DNA-protein crosslinks (DPCs), six indicators immune status, total hemocyte counts (THCs), differential (DHCs), hemocyanin (HC), prophenoloxidase (proPO), serine protease (SP) phenoloxidase (PO), were determined The results indicated that there was a significant increase (P
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