Adsorption of single DNA molecules at the water/fused-silica interface.
作者： Slavica Isailovic , Hung-Wing Li , Edward S. Yeung
DOI: 10.1016/J.CHROMA.2006.09.093
关键词:
摘要: We applied total internal reflection fluorescence microscopy (TIRFM) to study intermolecular interactions at the water/fused-silica interface single-molecule level. Real-time molecular motion was recorded reveal adsorption behavior and conformational dynamics of three DNAs with sticky ends different numbers unpaired bases. Features DNA interface, such as evanescent-field residence time, linear velocity frequency adsorption/desorption events were measured assess relative affinities oligonucleotides for surface. The general trend stronger interaction surface longer confirmed hydrophobic hydrogen bonding driving forces fused-silica pH 5. For sizes, accessibility give rise a nonlinear relationship respect affinity. Such information may prove valuable chromatography studies well design microarrays drug delivery systems.
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