Pangenomic study of Corynebacterium diphtheriae that provides insights into the genomic diversity of pathogenic isolates from cases of classical diphtheria, endocarditis, and pneumonia

摘要: Corynebacterium diphtheriae is one of the most prominent human pathogens and causative agent communicable disease diphtheria. The genomes 12 strains isolated from patients with classical diphtheria, endocarditis, pneumonia were completely sequenced annotated. Including genome C. NCTC 13129, we herewith present a comprehensive comparative analysis 13 first characterization pangenome species diphtheriae. Comparative genomics showed extensive synteny revealed core consisting 1,632 conserved genes. currently comprises 4,786 protein-coding regions increases at an average 65 unique genes per newly strain. Analysis prophages carrying diphtheria toxin gene tox that toxoid vaccine producer Park-Williams no. 8 has been lysogenized by two copies ωtox+ phage, whereas 31A harbors hitherto-unknown tox+ corynephage. DNA binding sites tox-controlling regulator DtxR detected genome-wide motif searches. content regulons exhibit marked differences due to gain, loss, partial deletion, site depletion. Most predicted pathogenicity islands characteristics horizontal transfer. majority these encode subunits adhesive pili, which can play important roles in adhesion different host tissues. All isolates contain least pilus clusters. It appears variation distributed common strategy establish host-pathogen interactions.