Multiplex Amplifiable Probe Hybridization (MAPH) methodology as an alternative to comparative genomic hybridization (CGH)

摘要: Genomic imbalances in locus copy-number are highly significant for the diagnosis and prognosis of cancer. Rapidly progressing DNA microarray technologies detect such pathogenic changes genome with high throughput, efficiency, resolution. A variety different microarray-based approaches have emerged, array comparative genomic hybridization (array-CGH) being method choice current clinical practice. Here we describe an alternative technique called array-MAPH, derived from conventional Multiplex Amplifiable Probe Hybridization (MAPH).The main novelty array-MAPH is directed reduction test complexity prior to hybridization, yielding a mixture specific probes, identical target sequences on thus increasing specificity. Unique amplifiable 400-600 bp fragments can be designed any region interest, PCR-amplified, spotted onto arrays as targets. The same combined into probe hybridized immobilized membrane. Bound probes recovered by quantitative PCR array. Array-MAPH used detection small-scale changes, thereby providing new insights genetic basis several diseases, including