Reversible alteration of calcium dynamics in cardiomyocytes during acute hypoxia transient in a microfluidic platform.

摘要: Heart disease is the leading cause of mortality in western countries. Apart from congenital and anatomical alterations, ischemia most common agent causing myocardial damage. During ischemia, a sudden decrease oxygen concentration alters cardiomyocyte function compromises cell survival. The calcium handling machinery, which regulates main functional features cardiomyocyte, heavily compromised during acute hypoxic events. Alterations dynamics have been linked to both short- long-term consequences ranging arrhythmias heart failure. In this perspective, we aimed at investigating cardiomyocytes early phase event. For purpose, developed microfluidic system specifically designed for controlling fast through gas micro-exchanger allowing line analysis intracellular by confocal microscopy. Experimental results show that exposure Fluo-4 loaded neonatal rat conditions induced changes Ca2+ transients. Such behavior was reversible detected levels below 5% partial pressure. observed were mimicked using specific L-type channel antagonists, suggesting alterations occur low levels. Reversible alteration ion function, takes place response cellular oxygen, might represent an adaptive mechanism cardiopreservation ischemia.