Investigation of sesquiterpene lactones as protein synthesis inhibitors of P-388 lymphocytic leukemia cells

摘要: The mode of action helenalin and bis(helenalinyl) malonate as protein synthesis inhibitors P-388 lymphocytic leukemia cells was investigated. initial characterizations were carried out in crude lysates the cells. In lysate, there a 4 min lag after addition drug before inhibition occurred. Both drugs allowed run-off preformed polysomes, but did significantly inhibit formation 80 S initiation complex suggesting preferential one or more reactions. effect these on both elongation reactions further investigated using fractionated systems prepared from Poly(U)-directed polyphenylalanine marginally inhibited by drugs, degree not sufficient to explain observed either lysate whole cell preparations P-388. ternary drug, conversion this 48 complexes inhibited. their